DYNAMIC
TEMPERATURE
CONTROL
Microscopic temperature control of your sample volume for high-resolution optical microscopy. Just set the temperature and start your measurement. Let VAHEAT do the rest.
Key Features
VAHEAT is a precise temperature control unit for optical microscopes. It combines local heating with direct temperature sensing in the sample volume. This allows for fast and precise temperature adjustment with heating rates up to 100°C/s while maintaining highest temperature precision. Made for investigations of temperature-sensitive processes in life sciences and material research.
Extreme temperature stability on long (hours to days) and short (seconds to minutes) time scales down to 0.01°C (rms). External temperature variations due to air flow, fluid exchange etc. will be detected and compensated via direct temperature feedback inside the sample volume.
Extend your experimental temperature range to 100°C (standard range) or even up to 200°C (extended range) depending on your experimental needs. The standard range version is compatible with oil-immersion systems while the extended range version can be operated with air objectives.
No optical aberration up to 80 °C with the highest numerical aperture objectives on the market. Perfectly suited for single molecule and super resolution studies using state-of- the-art methods (STORM, STED, TIRF, etc.). For more see Technical Performance.
VAHEAT lets you control the temperature inside the field of view independently from the type of microscope objective or the objective’s temperature. The system is designed as standalone unit without the need for any additional modifications to the optical setup (e.g objective heater) in order to avoid a temperature sink in your field of view. Additionally, the specific design of our smart substrates ensures that the objective’s performance is not altered even at higher temperatures.
VAHEAT is equipped with four heating modes for different experiments depending on your needs. Modes for fast heating, auto-compensated heating, or well-defined temperature profiles are available.
No thermal drifts or vibrations even at elevated temperature allow precise single molecule localization. We designed VAHEAT to be compatible with all commercial microscopes. No further modifications of your setup are needed. Its fast thermal response allows for nearly instantaneous thermalization tremendously reducing the waiting times as usual with conventional heating systems.
Extreme temperature stability on long (hours to days) and short (seconds to minutes) time scales down to 0.01°C (rms). External temperature variations due to air flow, fluid exchange etc. will be detected and compensated via direct temperature feedback inside the sample volume.
Extend your experimental temperature range to 100°C (standard range) or even up to 200°C (extended range) depending on your experimental needs. The standard range version is compatible with oil-immersion systems while the extended range version can be operated with air objectives.
No optical aberration up to 80 °C with the highest numerical aperture objectives on the market. Perfectly suited for single molecule and super resolution studies using state-of- the-art methods (STORM, STED, TIRF, etc.). For more see Technical Performance.
VAHEAT lets you control the temperature inside the field of view independently from the type of microscope objective or the objective’s temperature. The system is designed as standalone unit without the need for any additional modifications to the optical setup (e.g objective heater) in order to avoid a temperature sink in your field of view. Additionally, the specific design of our smart substrates ensures that the objective’s performance is not altered even at higher temperatures.
VAHEAT is equipped with four heating modes for different experiments depending on your needs. Modes for fast heating, auto-compensated heating, or well-defined temperature profiles are available.
No thermal drifts or vibrations even at elevated temperature allow precise single molecule localization. We designed VAHEAT to be compatible with all commercial microscopes. No further modifications of your setup are needed. Its fast thermal response allows for nearly instantaneous thermalization tremendously reducing the waiting times as usual with conventional heating systems.
Components
Control Unit
- STANDARD RANGE
- EXTENDED RANGE
Heating Power
<2500 mW
Max. temperature
105 °C.
Smart Substrates
SmS and SmS-R
Applications
High and super resolutions studies
The extended range version is meant for studies up to 200 °C. The maximal heating power of 5000 mW boosts your sample temperature to your desired setpoint even when larger thermal loads are attached. This system is made for investigations using air
spaced microscope objective for studying e.g. phase transitions or diffusional behavior.
Heating Power
<5000 mW
Max. temperature
200 °C
Smart Substrates
SmS, SmS-R and SmS-E
Applications
High resolutions studies
VAHEAT can be operated in four different modes that will ideally fit your experimental needs.
- AUTO
- DIRECT
- SHOCK
- PROFILE
The SHOCK mode resembles a timed DIRECT mode. For a defined period, a certain heating power will be applied to the sample volume without any feedback loop being active. This allows to exploit the fast heating dynamics of VAHEAT to send heat shocks into your sample.
Smart Substrates
Microscope Adapter
The heart of the VAHEAT system are the smart substrates that integrate a transparent thin film heating element with a highly sensitive temperature probe. The smart substrates with a heated area of 5 x 5 mm2 are inserted into the substrate holder that is connected to the control unit. As soon as the connector head establishes the connection between smart substrate and control unit, the temperature is being measured and you can start heating.
The microscope adapter fits into most of the common microscope stages due to its size of 75 mm x 25 mm (3” x 1”). Its slim design with a maximal height of 11 mm allows unrestricted access from above. The microscope adapter is thermally insulated from the heated area and stays at room temperature even for sample temperatures of 200 °C. Simply prepare your sample on the smart substrates, insert it into the microscope adapter and position it onto your translation stage to start your measurement. VAHEAT takes care of precise temperature control.
The User Interface
The platform independent user interface (UI) allows to remotely control the VAHEAT device, program arbitrary temperature profiles and stream temperature data to the local hard disk. You can use it for precise and live control of sample temperature and current heating power. Simply connect the control unit via USB to the computer and start the measurement remotely.
This is what our customers think about VAHEAT
Technical Performance
- IMAGING QUALITY
- TEMPERATURE STABILITY
- HEATING RATES
100x, NA=1.46, oil immersion objective
40x, NA=0.4, air objective
Temperature fluctuations over time
Temperature step response
Compatible Imaging Techniques
Total internal reflection microscopy (TIRM)
Confocal microscopy
Interferometric scattering microscopy (iSCAT)
Atomic force microscopy (AFM)
Super resolution methods (SIM, STORM, PALM, PAINT, STED)
Widefield microscopy
Total internal reflection microscopy (TIRM)
Confocal microscopy
Interferometric scattering microscopy (iSCAT)
Atomic force microscopy (AFM)
Super resolution methods (SIM, STORM, PALM, PAINT, STED)
Widefield microscopy
Application Examples
- LIVE CELL IMAGING
- DNA
- MICROFLUIDICS
- PHASE TRANSITIONS
- NEUROSCIENCE
- AFM
Living organisms are highly sensitive to changes in their environmental conditions, especially to temperature. VAHEAT ensures reliable and precise temperature control while transfer and during imaging. Investigating temperature sensitive processes of cellular behavior, such as Ca2+ activity in multicellular tumor spheroids or thermal stimulation of neurons has never been this easy.
Exploit VAHEAT ‘s capabilities as micro stage top incubator.
CAtegories:
Cell Biology | Neuroscience | Medicine | Stage Top Incubator
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CAtegories:
Nanotechnology | Biochemistry | Biotechnology
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Videos
VAHWAT as stage top incubator for live cell imaging
Long-term live cell imaging of normal human dermal fibroblasts (NDHF) that are stained with SIR-tubulin. The cells are incubated using only a Smart Substrate with Reservoir (SmS-R) and are continuously imaged for more than 18 hrs. No need for large incubation chambers, objectives heater or prior setup calibration!
Heat shock of live cells
Human dermal fibroblasts (NDHF) that are stained with SIR-tubulin are held at 37 °C using VAHEAT as a stage top incubator. The sample volume is continuously imaged while sending heat shocks of 45°C for 30 s and 55°C for 30 s into the sample that result in cell death. The imaging quality is maintained during and the sample position is not shifted.
Web seminar on temperature control in high-resolution microscopy
Watch our web seminar recorded on 18 th November 2020. Invited speakers are Mart Krupovics (Institut Pasteur, Paris) and Phillip Tripal (Optical Imaging Center, Erlangen).
Steering a crystallization process
A small crystal of Phenyl 1-hydroxy-2-naphthoate is imaged with a upright microscope while the temperature is being adjusted using VAHEAT.
Temperature control with VAHEAT
Inverted microscope with oil immersion objective is equipped with a VAHEAT system. Only the smart substrate is temperature controlled (no objective heater). The infrared camera shows a homogeneous temperature distribution inside the heated area although the objective is in thermal contact with the smart substrate.
FAQs
Content
The standard range version can issue heating powers up to 2.5 W and is working with smart substrates of 170 μm thickness (#1.5H) as required for high NA immersion microscope objectives. The high-temperature version can be operated with smart substrates of 500 μm thickness (#5) while allowing heating powers of up to 5.0 W.
Yes! VAHEAT is compatible with vacuum chambers. You might require a specific cable feedthrough but there will be no outgassing when operating VAHEAT inside a vacuum chamber. Contact us if you need more information.
Yes! We specifically designed VAHEAT
for high- and super-resolution microscopy. The smart substrates have a thickness of 170 μm and are thus compatible with almost any commercially available high Numerical Aperture (NA) objective needed for super- resolution microscopy.
The conventional thermal problems that arise in super-resolution microscopy when working at elevated temperature are fully avoided when working with VAHEAT. We also measured the optical performance with a high NA objective as function of temperature. You can check out the data here.
You cannot believe this? Get a demo system and test it yourself!
From internal measurements we can conclude that the sample heating has no effect on the image quality and therefore on the point spread function (PSF) for temperatures between room temperature and 100 °C.
We recommend oil and water based immersion medium. Please avoid glycerine as immersion medium as it will attack the smart substrates and depreciate their functionality.
Yes! We offer a five-week test period. Reach out to us!
