VAHEAT as a precise temperature reference during imaging

Webinar, Wednesday, March 23rd, 5 p.m. CET

Everything in nature is in one way or another dependent on temperature. That’s why scientific experiments need precise temperature control and calibration. In this webinar, Jaroslav Icha from Interherence will briefly talk about our miniaturized stage top incubator VAHEAT, which is the only device that controls the temperature directly in the field-of-view. Our three academic speakers will introduce examples of experiments from their research, which benefited from precise temperature calibration with VAHEAT.

Andrew York (Calico, USA) will talk about a completely new type of fluorescent biosensors that his team has developed. These protein sensors are based on measuring fluorescence relaxation. The relaxation rate depends besides the measured quantity, like pH, also on temperature. It is important to calibrate their response to extract meaningful and reproducible data from these experiments. Then, the relaxation sensors present a very powerful technology especially for in vivo applications. Here you can learn more about this project.

Guillaume Baffou (Institut Fresnel, France) will present experiments with thermobleaching and photobleaching of nanoparticles that were carried out during a project focused on microscale thermophoresis in liquids assisted by laser heating of gold nanoparticles. Additionally, he will present work in progress focused on measuring temperature-dependent refractive index changes in liquids. Here you can learn more about the thermophoresis project.

Pascal Lorentz (University of Basel, Switzerland) will be talking about using VAHEAT as a temperature calibration tool in the context of a light microscopy facility.

Sign up to secure your spot, the number of live attendees is limited. The event is free.

Andrew York Calico using VAHEAT for fluorescent relaxation biosensors

Andrew York (Calico): “Fluorescence relaxation-based sensors”

Andrew is a physicist with a background in optics, a passion for invention and paradox, and no training in biology. The York Lab uses math, physics, engineering and coding to make biological imaging faster, gentler, higher resolution and more useful. Whenever possible, they share their work in real-time as “open science”; see andrewgyork.github.io for recent publications. Before joining Calico, Andrew invented the technology behind all-optical superresolution microscopes like the Yokogawa SoRa and the VisiTech iSIM. At Calico, the York lab developed the “Snouty” lens, that enables light-sheet microscopy with no compromise in resolution or sample geometry.

Guillaume Baffou (Institut Fresnel): “VAHEAT for metrology of refractive indices of liquids and photo/thermobleaching of fluorophores”

Guillaume is currently a CNRS researcher working at the Institut Fresnel, Marseille (France). He graduated from ENS Paris-Saclay, received his master’s degree in solid states physics from Université Paris XI in 2004 and his Ph.D. degree in Nanoscience at the same place in 2007. With a 3-year long postdoc on plasmonics and associated photothermal effects, he was then appointed as CNRS research at Institut Fresnel in 2010. After being awarded the Bronze Medal of the CNRS in 2015, he obtained an ERC Consolidator grant to lead research activities at the interface between physics and biology at small scales.

 

Pascal Lorentz using VAHEAT for temperature calibration in microscopy

Pascal Lorentz (University of Basel): “VAHEAT as a measuring device”

Pascal grew up and studied in Basel. In his research, he explored the role of growth factors in tumorigenesis based on transgenic mouse models in the Group of Gerhard Christofori.
In 2007, he got the chance to build up and lead the microscopy core facility at the Department of Biomedicine. Since then, the facility grew quite significantly and is currently offering around 30 high end systems and serves about 270 users.

 

The number of live attendees is limited.
The event is free.